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Phosphoinositides, especially PI(3,4,5)P3, and PI(4,5)P2, are recognized by SHIP2 a member of the inositol polyphosphate 5-phosphatase family. In different glioblastoma cell lines and primary cultures, SHIP2 discoloration in the plasma membrane partially contrasts with PI(4,5)P2 immunoreactivity. Through depletion of SHIP2 in a glioblastoma cell line 1321 N1 cells, we now reveal that SHIP2 inhibits cell regeneration. Thusour results show a novel role of SHIP2 in the control of PI(4,5)P2, PI4P and cell migration in PTEN-deficient glioblastoma N1 cells. Regulation of PI(4,5)P2 and PI4P content by SHIP2 controls N1 cell migration through the company of focal adhesions.
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